Materials and Methods. All animal experiments were conducted in accordance with the guidelines and approval of the Animal Research and Ethics Committees of Sun Yat-sen University. Grouper were cultivated in the Marine Fisheries Development Center of Guangdong Province. In April 2015, 100 groupers weighing approximately 5 kg were sacrificed for tissue sampling after being anesthetized by MS222. For each fish, the gonad and brain were taken and stored at −80°C for RNA extraction, and a piece of gonadal tissue was fixed in Bouin’s fluid for histological examination. Genome-Wide Identification of β -hsd Genes. To identify candidate β -hsd genes in these thirteen species, we collected the gene and protein names of the β -hsd gene family from the literature (Payne et al., 1997; Dai et al., 2003; Mindnich et al., 2004; Seckl, 2004; Hoffmann and Maser, 2007; Meier et al., 2009; Mindnich and Adamski, 2009; Sreenivasulu and Senthilkumaran, 2009; Rotinen et al., 2010; Kisiela et al., 2012; Saloniemi et al., 2012; Tokarz et al., 2012; Lin et al., 2013; He et al., 2018).
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The introduction of glucocorticoid replacement and increased awareness have increased the survival of classic 21OHD [5], and this is most probably also the case in 3βHSD2D. In population studies, patients with CAH had generally an increased mortality rate (hazard ratio 3–5) and died 6.5–18 years earlier, compared to controls [122, 135]. Adrenal crisis was the main cause of death [135], iterating the importance of stress dosing during acute illness [40, 41]. Mortality studies in pure 3βHSD2D will probably never be performed due to its rareness. Glucocorticoid and mineralocorticoid replacement is similar to other forms of CAH. In SW crisis, treatment includes prompt rehydration, correction of hypoglycemia, and parenteral hydrocortisone (intravenous or intramuscular) [15, 40, 41]. Casa de aposta dando bônus sem depósito.
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